5-Channel Fluorescence Imaging Simplified
Increasing the number of parameters that can be measured with fluorescence imaging is a powerful means of increasing the information an experiment can provide. It also minimizes the number of experiments required to acquire a given amount of information. New, extremely bright fluorochromes of a new class of fluorescent molecule from BD Biosciences enables 5-color imaging, from violet to far-red fluors like Cy5 with a basic microscope stand, light source, and camera.
For most workers who are not imaging specialists, acquiring data from 4 or 5 fluorescence channels has not been easily achieved.
Here, Chroma Technology in collaboration with Becton Dickinson Biosciences proposes an imaging scheme for imaging with 5 fluorescence channels with very basic equipment and imaging skills. Therefore reliable multiplexing is now possible by the non-specialist.
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