Enhancing Two-Photon Microscopy

Enhancing Two-Photon Microscopy

Two-photon microscopy has many advantages over conventional microscopy including: minimal photo-bleaching effect and sample damage, higher imaging depth of live cells, higher excitation efficiency, and simultaneous excitation of multiple fluorophores with the promotion of autofluorescence.

Ultrashort (<10 fs) pulses enhance the two-photon microscopy technique. The peak power for a given average output is much higher in ultrashort pulses, resulting in reduced scattering, and thus leading to deeper imaging, higher fluorescence efficiency and improved image contrast. As the pulses enable a highly localised excitation of the sample, this reduces sample exposure to damaging energy, meaning reduced photo-bleaching.

Having a broad spectral output covering the complete Ti:Sapphire emission range between 640 nm and 1100 nm allows for multiple dye excitation and the promotion of autofluorescence without needing a tuneable laser — an important factor for those looking at live biological samples.

The venteon ultra produced by Laser Quantum brings the capability of <5 fs transform-limited pulses thus offering the benefits of enhanced excitation efficiency and direct excitation of multiple fluorophores due to the broad spectral output.

For more information about the venteon ultra, click here.

 

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