Achieving Higher Resolution and Faster Sampling in Microscopy
Fluorescence microscopy is a continually developing tool for imaging biological samples; however, the same challenges face the entire the microscopy world:
- High-resolution levels
- Faster sampling rates
Lattice light-sheet microscopy (LLSM) is a technique that employs scanning a sample as a plane of laser light. A second excitation lens is placed on a perpendicular axis to the detection lens. Light-sheet microscopy can therefore enable faster acquisition speeds and minimize phototoxicity and photobleaching effects. As another benefit, light-sheet microscopy improves this aspect through careful selection and tuning of the period of the lattice, filtering out unwanted frequencies, therefore offering an improved axial resolution.
Suitable source lasers for LLSM are necessary across a range of visible wavelengths for excitation of different fluorophores. They must offer very good beam quality and highly stable output power over long and short time scales. Laser Quantum produces the gem series of solid-state CW fibre-coupled lasers that offer excellent beam spatial profile (M2 <1.1), beam pointing stability (<10 µRad/°C) and output power stability specifications (<0.8% RMS over 100 hours at constant temperature) in an exceptionally robust, compact package.
For more details about light-sheet microscopy, click here.
To request more information or a quotation for this or other Laser Quantum products, contact IL Photonics.