Advanced Analysis of Single-Molecule Spectroscopic Data
This post highlights Chroma filters in a unique role — software development.
Full SMS is a cutting-edge, GUI-based application for single-molecule spectroscopy (SMS) data analysis, making advanced research more accessible and efficient. A new paper, entitled “Advanced analysis of single-molecule spectroscopic data,” presents Full SMS.
Here is the Abstract of the paper:
We present Full SMS, a multipurpose graphical user interface (GUI)-based software package for analysing single-molecule spectroscopy (SMS) data. SMS typically delivers multi-parameter data — such as fluorescence brightness, lifetime, and spectra — of molecular or nanometre-scale particles such as single dye molecules, quantum dots, or fluorescently labelled biological macromolecules. Full SMS allows an unbiased statistical analysis of fluorescence brightness through level resolution and clustering, analysis of fluorescence lifetimes through decay fitting, as well as the calculation of second-order correlation functions and the display of fluorescence spectra and raster-scan images. Additional features include extensive data filtering options, a custom HDF5-based file format, and flexible data export options. The software is open source and written in Python but GUI-based so it may be used without any programming knowledge. A multi-process architecture was employed for computational efficiency. The software is also designed to be easily extendable to include additional import data types and analysis capabilities.
The experimental setup for this research used the following Chroma components:
About Chroma Technology
Chroma Technology is a leading manufacturer and OEM supplier of high performance optical filters used in a wide range of applications, including health care, biomedical research, remote sensing, machine vision, and aerospace. Chroma offers an extensive selection of optical filter sets and filter accessories, as well as custom solutions tailored to customer-specific needs.
For more information or a quotation for this or other Chroma products, contact IL Photonics.