Brillouin Light Scattering Microscopy Used in Cytoskeletal Research
A recent article in “Nature,” entitled “Cytoskeletal disorganization underlies PABPN1-mediated myogenic disability” suggests that “poor cytoskeletal mechanical features are caused by altered expression levels of cytoskeletal proteins and contribute to muscle wasting and atrophy.”
This research involved Brillouin light scattering microscopy using a home-built Brillouin confocal microscope. A single mode torus 532 nm laser from Laser Quantum performed the excitation for this microscope. For all scans, the laser power at the sample was between 1–3 mW, and the swell time per point, which was also the acquisition time of each spectrum, was 100 ms.
The torus 532 laser is a high specification, green single frequency laser. Using intelligent electronics, the torus continually tracks its longitudinal mode position and ensures there is no mode-hop. The torus is available at 532 nm, with powers ranging from 50–750 mW, making it ideal for applications such as holography, Brillouin scattering and Raman spectroscopy. The torus is a single longitudinal mode laser. Control of the laser can be accessed through a dedicated RS232 port on the PSU. The unique patented travelling-wave cavity results in an ultra-low noise output.
To request more information or a quotation for this or other Laser Quantum products, contact IL Photonics.