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Total Internal Reflection Fluorescence (TIRF) microscopy restricts excitation to a thin layer near the sample surface, often less than 100 nanometers. This technique dramatically improves signal-to-noise ratio for surface-proximal imaging. That performance depends on filters and dichroics that can handle excitation energy re-entering the emission path at high angles, something standard fluorescence optics aren’t built … Continue reading
Overview A customer developing a multispectral fluorescence imaging system for biological tissue needed custom optical filters capable of resolving seven fluorescent dyes across eight image volumes. Engaged from the beginning of the project, Chroma Technology provided initial proposals, modeled spectral curves alongside, and refined the filter set through iterative design reviews over two years. Close … Continue reading
Confocal microscopy puts unusual demands on optical filters, where each filter type in the light path has its own job to do. Excitation filters clean up the laser line before it reaches the specimen, typically as a narrow ~10 nm bandpass. Dichroic mirrors need high flatness to keep the beam quality intact under high laser … Continue reading
Chroma Technology collaborated with a sensor manufacturer in the oil and gas industry that was facing challenges in contaminant detection within pipelines. By designing custom short-wave infrared (SWIR) optical filters with precise band widths, cut-ons, and cut-offs — and optimizing coatings for durability — Chroma enabled the sensor to effectively isolate chemical signatures in field … Continue reading
In the world of semiconductor manufacturing, where nanoscale precision can make or break a product, optical filters play a crucial yet often overlooked role. Chroma Technology understands that the quality of your optical filters directly impacts the accuracy of your inspection processes and ultimately, the performance of your semiconductors. Chroma specializes in creating advanced optical … Continue reading
Two new SHO-based filter sets have just been added to the Chroma Technology astronomy catalog. The 27111 – 8 nm SHO Set brings together matched sulfur II, hydrogen-alpha, and oxygen III filters for capturing emission nebulae, planetary nebulae, and supernova remnants. The 27112 – 8 nm SHO + LRGB Set adds a full luminance, red, … Continue reading