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Light-sheet fluorescence microscopy (LSM) is now an important imaging technique in the biological sciences. This method can provide rapid volumetric imaging with minimal photo damage. Even though LSM is suited for in-vivo imaging such as endomicroscopy and neural imaging in freely moving animals, translation to miniaturized optical systems has been limited to date. A paper … Continue reading
The application of endomicroscopes in various biomedical fields such as neuroscience and cancer detection and treatment especially of inner, hardly accessible organs demands the smallest possible diameters in combination with a large numerical aperture (NA) and field-of-view (FOV). Here are some excerpts from the Introduction to the paper, entitled “Field curvature reduction in miniaturized high … Continue reading
Multimodal non-linear microscopy, combining coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), and two-photon excited fluorescence (TPEF), provides non-invasive and label-free information on the molecular composition and morphology of biological specimens ex vivo, enabling the detection of diseases. A paper describing the development of such a detection system is entitled, “Multimodal nonlinear endomicroscopic imaging … Continue reading
A recently published paper describes how gradient index (GRIN) lenses are used to perform microendoscopy on brain neurons. This paper is entitled, “High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo.” The Abstract of this paper states: Optical imaging has become a powerful tool for studying brains in vivo. The opacity of adult … Continue reading