Explore New Depths in Microscopy with CRONUS
Building on over 25 years of experience in designing and manufacturing femtosecond lasers and wavelength-tunable sources, Light Conversion has recently introduced CRONUS-2P and CRONUS-3P microscopy-dedicated femtosecond laser sources covering applications in functional neuroimaging, optogenetics, and deep imaging using medium-repetition-rate, three-photon excitation and fast high-repetition-rate, two-photon imaging.
CRONUS-3P provides μJ-level pulses down to 50 fs at repetition rates of up to 2 MHz and tunable in the short wavelength infrared (SWIR) range from 1.25 to 1.8 μm, thus covering the biological transparency windows at 1.3 μm and 1.7 μm for three-photon microscopy. Typically, multiphoton imaging in the SWIR range requires a complex multi-device laser system and usually a large part of an optical table and skilled staff. This combination creates a reality that encumbers neuroscience and other biomedical applications. The CRONUS-3P system is a next-generation, industrial-grade, single-supplier solution that is more compact, more reliable, and more versatile. This system is therefore able to achieve tunable femtosecond excitation with an integrated Group Delay Dispersion (GDD) control. This ensures optimal pulse duration at the sample, as well as industrial-grade design to guarantee excellent short- and long-term output stability.
CRONUS-2P is a femtosecond laser providing three simultaneous synchronized outputs with high repetition rate, high output power, short pulse duration, and GDD control, making it the ultimate source for nonlinear microscopy. Two outputs are independently tunable in the 680–960 nm and 960–1300 nm ranges, while the third is fixed at 1025 nm. Uses of the CRONUS-2P include simultaneous excitation of multiple fluorescent probes, calcium indicators, or opsins at their absorption maxima. In addition, second- and third-harmonic emission (SHG and THG) can be spectrally shifted for either ease of detection or resonant enhancement. The three simultaneous outputs also provide for advanced coherent anti-Stokes and stimulated Raman scattering (CARS and SRS) applications with dual-band imaging, a broader selection of vibrational resonance frequencies, constant-difference dual-beam tuning, resonant enhancement, and more.
To request more information or a quotation for this or other Light Conversion products, contact IL Photonics.