Filters for Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR) is a technique to make multiple copies of a short sequence of target DNA, exponentially amplifying it. DNA must be amplified so that there is enough material to reliably detect and identify it with a high degree of confidence. The multitude of PCR applications include infectious disease diagnosis.
If the target DNA sequence from a pathogen of interest is detected in a sample from a patient and subsequently amplified, its presence is confirmed by a fluorescence signal. If the target is an RNA sequence, then DNA that is complementary to the RNA is amplified, as is the case with RNA viruses such as the coronavirus responsible for COVID-19.
The goal of PCR is to make enough of the target DNA region so that its presence can be measured or confirmed for diagnostic, clinical, or experimental purposes. The value of this approach is that only the specific target sequences must be amplified, without needlessly amplifying all the DNA in an entire gene or chromosome.
Chroma Technology supplies key components of PCR laboratory and clinical test solutions with their line of sputter-coated optical filters for PCR applications. They have been supplying high-quality PCR filters to major test equipment manufacturers for many years. This has provided Chroma with valuable experience and application expertise. Their technical staff not only have a deep understanding of fluorescence, managing light, and biophotonics, but also have extensive experience working with PCR investigators and engineers.
For more information or a quotation for any Chroma products, contact IL Photonics.