For TIRF Imaging ― Make Your World Flat
If you are imaging in Total Internal Reflection Fluorescence (TIRF) mode, your world must be very flat. Even very slight curvature or flexing of the surface of a dichroic used to reflect a TIRF laser results in astigmatism. This distorts the reflected laser beam ― and that results in a less-than-optimal evanescent wave for your imaging. This distortion also results in laser photons propagating into your sample, producing spurious fluorescence beyond the TIRF zone.
Chroma Technology’s complete, sputtered TIRF filter sets, mounted into their patented metal TIRF cubes, provide three unique advantages to optimize your TIRF data:
- Create optimal evanescent waves with Chroma’s UltraflatTM dichroics, which allow for distortion-free reflection or TIRF lasers.
- Keep your world flat with Chroma’s patented metal filter cubes (available for most current microscope models).
- Maximize the signal-to-noise ratios of your images with emission filters that provide higher optical density.
To read the TIRF Filter Cubes data sheet, click here.
To request more information or a quotation for Chroma products, contact IL Photonics.