greateyes Camera Used in Correlative Microscopy Study
Learn about an application of the GE-VAC In-Vacuum Camera produced by greateyes in a recent study published in the journal “Microscopy and Microanalysis.” The article describing this study is entitled “Laboratory-Based Correlative Soft X-ray and Fluorescence Microscopy in an Integrated Setup.”
Here is a synopsis of the article:
Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional contrast by imaging only specifically labeled components, especially in biological samples. However, the achievable structural information on the sample in its full complexity is limited. Here, the intrinsic label-free carbon contrast of water window soft X-ray microscopy can complement fluorescence images in a correlative approach ultimately combining nanoscale structural resolution with functional contrast. However, soft X-ray microscopes are complex and elaborate, and typically require a large-scale synchrotron radiation source due to the demanding photon flux requirements. Yet, with modern high-power lasers it has become possible to generate sufficient photon flux from laser-produced plasmas, thus enabling laboratory-based setups. Here, we present a compact table-top soft X-ray microscope with an integrated epifluorescence modality for ‘in-situ’ correlative imaging. Samples remain in place when switching between modalities, ensuring identical measurement conditions and avoiding sample alteration or destruction. We demonstrate our new method by multimodal images of several exemplary samples ranging from nanoparticles to various multicolor labeled cell types. A structural resolution of down to 50 nm was reached.
About greateyes Cameras
greateyes offers a series of high-performance, cooled, full-frame, CCD scientific cameras for imaging and spectroscopy in the range of X-ray to near-infrared (NIR). The cameras combine scientific, highly sensitive CCD sensors and ultra-low noise electronics for optimal detection of weak signals and measurements with high dynamic range. They can be used as detectors for a broad range of applications common in the industrial and scientific community, including:
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