High Resolution Optical Imaging Deep in Living Tissue
Breakthroughs in imaging technology have been the primary driving force of new biological discoveries. However, up to now, high-resolution optical imaging deep in tissues was challenging because of optical aberrations and scattering of light caused by the complex structure of living matter.
Here, researchers from the the Hong Kong University of Science and Technology present an adaptive optics three-photon (3P) microscope, based on analog lock-in phase detection for focus sensing and shaping (ALPHA-FSS), to accurately measure and compensate for both aberrations and scattering induced by specimens and to recover subcellular resolution at depth. The microscope employed ORPHEUS-F short-pulse excitation at 1300 nm, enabling imaging up to 1.1 mm below the pia within the intact brain.
To read the article entitled, “Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping,” published by this research team, click here.
- Combination of the best collinear and non-collinear OPA features
- 650–900 nm and 1200–2500 nm tuning range
- Single-shot, 2 MHz repetition rate
- < 100 fs pulse duration
- Adjustable spectral bandwidth
- Optional long pulse mode for gap-free tunability
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