Improving Imaging Experiments by Combining LEDs with Optical Filters
Combining state-of-the-art LED illumination with innovative optical filter configurations can significantly improve the quality and efficiency of your imaging experiments.
When choosing filter sets for your experiments, it’s not just the optical properties of your fluorophores you need to consider. To enhance speed and contrast, the latest microscope technology has also introduced options in terms of where to position these filters — and this is no longer limited to the filter cube.
Learn how these technologies work together to achieve precise, high-speed fluorescence imaging. An article explores the pros and cons of a few different options for combining the latest technology in widefield fluorescence, comparing various filter types. Topics in this article include:
- How different filter configurations affect speed and contrast
- LEDs – the go-to illumination technology for widefield fluorescence
- Pinkel and Sedat considerations
- Multi-Sedat hybrid sets considerations
- The future of high-speed multi-color imaging
About Chroma Technology
Chroma Technology is a leading manufacturer and OEM supplier of high performance optical filters used in a wide range of applications, including health care, biomedical research, remote sensing, machine vision, and aerospace. Chroma offers an extensive selection of optical filter sets and filter accessories, as well as custom solutions tailored to customer-specific needs.
For more information or a quotation for this or other Chroma products, contact IL Photonics.

