Introducing the RUSH3D Mesoscope
RUSH3D is a compact real-time, ultra-large scale, high-resolution 3D mesoscope, providing a 13x improvement in data throughput and a significant reduction in system size and cost.
Features of the RUSH3D mesoscope include:
- RUSH3D allows long-term, fast 3D imaging at centimeter-scale and single-cell resolution.
- Compact, high-fidelity mesoscale imaging is achieved in native intravital environments.
- Intercellular interactions across multiple mouse germinal centers are observed in vivo.
- Cortex-wide multisensory, locomotion neural activities and immune responses are explored in mice.
The paper describing this research is entitled, “Long-term mesoscale imaging of 3D intercellular dynamics across a mammalian organ.” The Summary statement for this paper states:
A comprehensive understanding of physio-pathological processes necessitates non-invasive intravital three-dimensional (3D) imaging over varying spatial and temporal scales. However, huge data throughput, optical heterogeneity, surface irregularity, and phototoxicity pose great challenges, leading to an inevitable trade-off between volume size, resolution, speed, sample health, and system complexity. Here, we introduce a compact real-time, ultra-large-scale, high-resolution 3D mesoscope (RUSH3D), achieving uniform resolutions of 2.6 × 2.6 × 6 μm3 across a volume of 8,000 × 6,000 × 400 μm3 at 20 Hz with low phototoxicity. Through the integration of multiple computational imaging techniques, RUSH3D facilitates a 13-fold improvement in data throughput and an orders-of-magnitude reduction in system size and cost. With these advantages, we observed premovement neural activity and cross-day visual representational drift across the mouse cortex, the formation and progression of multiple germinal centers in mouse inguinal lymph nodes, and heterogeneous immune responses following traumatic brain injury—all at single-cell resolution, opening up a horizon for intravital mesoscale study of large-scale intercellular interactions at the organ level.
This mesoscope comprises products from Chroma Technology and 89 North’s LDI. The Chroma items include:
- ZET405/488/561/640xv2 dichroic mirror
- ZET405/488/561/640xv2 excitation filter
- ZT405/488/561/640mv2 emission filter
About Chroma Technology
Chroma Technology is a leading manufacturer and OEM supplier of high performance optical filters used in a wide range of applications, including health care, biomedical research, remote sensing, machine vision, and aerospace. Chroma offers an extensive selection of optical filter sets and filter accessories, as well as custom solutions tailored to customer-specific needs.
For more information or a quotation for this or other Chroma products, contact IL Photonics.