Introducing Two New Yellow Fluorescent Proteins
A new study introduces two yellow fluorescent proteins (YFPs) — mGold2s and mGold2t — that are significantly more photostable than commonly used alternatives such as mVenus and mCitrine.
Using these new YFPs, researchers were able to extend imaging durations across diverse modalities including widefield, total internal reflection fluorescence (TIRF), super-resolution, single-molecule, and laser-scanning confocal microscopy.
The paper is entitled, “Bright and photostable yellow fluorescent proteins for extended imaging.”
Hardware Setup for the Research from Chroma Technology and 89 North.
Hardware for SPOTlight Screening
Cyan, yellow, and red FPs were excited with 445/3-nm (peak/bandwidth), 520/5-nm, and 555/0.6-nm light, respectively, from a laser diode illuminator (LDI-WF from 89 North). Multi-bandpass dichroic mirrors (for CFP and YFP: ZT445/520rpc, Chroma; for RFP: ZT 405/470/555/640rpc, Chroma) were used. Excitation light was filtered with multiband filters (for CFP and YFP: ZET445/520x, Chroma; for RFP: ZET 405/470/555/640x, Chroma). Emission light was filtered with multiband filters (for CFP and YFP: ZET445/520M-OD8, Chroma; for RFP: ZET 405/470/555/640 m, Chroma).
Hardware for Determining 2P Photostability
The emission light from the cell was split using a 560-nm dichroic mirror (348958, Chroma), filtered by a 525/50-nm bandpass filter (353716, Chroma) and collected by a gallium arsenide phosphide (GaAsP) photomultipliers tube (PMT).
Hardware Determining 2P Brightness
The emission light from the cell was split using a 560-nm dichroic mirror (348958, Chroma), filtered by a 525/50-nm (353716, Chroma) and a 440/80-nm (Chroma) bandpass filter for the yellow and blue channel, respectively, and collected by gallium arsenide phosphide (GaAsP) photomultipliers tubes (PMTs).
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About Chroma Technology
Chroma Technology is a leading manufacturer and OEM supplier of high performance optical filters used in a wide range of applications, including health care, biomedical research, remote sensing, machine vision, and aerospace. Chroma offers an extensive selection of optical filter sets and filter accessories, as well as custom solutions tailored to customer-specific needs.
For more information or a quotation for this or other Chroma products, contact IL Photonics.