For TIRF Imaging ― Make Your World Flat
If you are imaging in Total Internal Reflection Fluorescence (TIRF) mode, your world must be very flat. Even very slight curvature or flexing of the surface of a dichroic used to reflect a TIRF laser results in astigmatism. This distorts the reflected laser beam ― and that results in a less-than-optimal evanescent wave for your … Continue reading