Tag Archives: fluorescence microscopy

An Introduction to Fluorescence Imaging

Imaging microscopic biological specimens demands greater resolution than can typically be attained via conventional microscopy. Consider a cell, which is predominantly composed of water and is subsequently transparent. Visible light-based microscopy will fail to provide much detail about cellular structures, as most light on the range of 400–700 nanometers (nm) will simply transmit through. This … Continue reading

December 30, 2020 Blog, Laser Quantum, Lasers

Cost-Efficient Open Source Laser Engine for Microscopy

In a paper published in Biomedical Optics Express, entitled “Cost-efficient open source laser engine for microscopy”, the authors explain how they developed a cost-efficient, open source laser engine for fluorescence microscopy. Their experimental setup included a powerful diode-pumped solid-state laser (DPSS) gem 561 laser (561 nm, 500 mW) from Laser Quantum. To read the full … Continue reading

August 18, 2020 Blog, Laser Quantum, Lasers

Fluorescence Multiplexing in Microscopy Imaging and Flow Cytometry

Fluorescence multiplexing itself is not new, but its power to acquire large amounts of relevant biological information quickly has advanced significantly in the last few years. Do you have questions about multiplexing? Despite its usefulness to correlate multiple elements, many are unfamiliar with recent advances which are expanding application of this technique. That is why … Continue reading

January 15, 2020 Blog, Cameras and Imaging, Chroma Technology, Spectroscopy

HiCAM Fluo with SCAPE Microscopy Enables Real-Time In-Vivo Dynamics

Lambert Instruments is pleased to recognize the work described in a recent Nature Methods article authored by the research group of Professor Elizabeth Hillman at Columbia University in the City of New York. The researchers used SCAPE microscopy in combination with the Lambert Instruments HiCAM Fluo to achieve high-resolution volumetric imaging at over 300 volumes-per-second … Continue reading

October 2, 2019 Blog, Cameras and Imaging, Lambert Instruments

Achieving Higher Resolution and Faster Sampling in Microscopy

Fluorescence microscopy is a continually developing tool for imaging biological samples; however, the same challenges face the entire the microscopy world: High-resolution levels Faster sampling rates Lattice light-sheet microscopy (LLSM) is a technique that employs scanning a sample as a plane of laser light. A second excitation lens is placed on a perpendicular axis to … Continue reading

September 25, 2019 Blog, Laser Quantum, Lasers